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prc2 mi342 plasmids  (TaKaRa)


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    Structured Review

    TaKaRa prc2 mi342 plasmids
    Prc2 Mi342 Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prc2+mi342+plasmids/AAVpro+Helper+Free+System/bio_rxiv__64898__2026__04__30__721990-138-13-15
    Average 94 stars, based on 12 article reviews
    prc2 mi342 plasmids - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma.
    Article Snippet: The pAAV-nEFCas9 (Addgene, plasmid 87115) was used to express Cas9, and the pAAV-tagBFP U6-gRNA expression vector was used to express a gRNA targeting the start codon of cMYC: (5′ GACGTTGAGGGGCATCGTCGC 3′). .. Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Three days after transfection, cells were harvested, and AAV2 was isolated using AAVpro D ow nloaded from https://academ ic.oup.com /genetics/article/224/3/iyad083/7147209 by guest on 14 Septem ber 2024 Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma.
    Article Snippet: The presence of the correctly tagged MYC protein was validated by western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested 3 days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The pAAV-nEFCas9 (Addgene, plasmid 87115) was used to express Cas9, and the pAAV-tagBFP U6-gRNA expression vector was used to express a gRNA targeting the start codon of cMYC: (5’ GACGTTGAGGGGCATCGTCGC ’). .. Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Three days after transfection, cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The pAAV-nEFCas9 (Addgene, plasmid 87115) was used to express Cas9, and the pAAV-tagBFP U6-gRNA expression vector was used to express a gRNA targeting the start codon of cMYC: (5’ GACGTTGAGGGGCATCGTCGC 3’). .. Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Three days after transfection, cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Changes in the Transcriptome and Synthetic Lethal Dependencies Following KRAS Mutant Expression Reveal Profound Tissue-Specificity
    Article Snippet: .. Recombinant AAV serotype 2 was packaged in 293T cells using the pHelper and pRC2-mi342 plasmids (Takara cat# 6652). .. A 10-cm plate of 293T cells was transfected with 5 μg pHelper, 5 μg pRC-mi342, and 5 μg LSL-3xHA KRAS targeting vector plus 45 μL PolyJet transfection reagent (SignaGen cat# SL100688).

    Article Title: cMYC protein interactions and chromatin association in NUT carcinoma
    Article Snippet: The pAAV-nEFCas9 (Addgene, plasmid 87115) was used to express Cas9, and the pAAV-tagBFP U6-gRNA expression vector was used to express a gRNA targeting the start codon of cMYC: gRNA-cMYC-start ( ). .. Recombinant AAV2 was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Three days after transfection, cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Plasmid Preparation:

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma.
    Article Snippet: The presence of the correctly tagged MYC protein was validated by western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested 3 days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Article Title: Cell state-dependent chromatin targeting in NUT carcinoma
    Article Snippet: The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). .. The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). .. Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235).

    Bioprocessing:

    Article Title: Changes in the Transcriptome and Synthetic Lethal Dependencies Following KRAS Mutant Expression Reveal Profound Tissue-Specificity
    Article Snippet: .. Recombinant AAV serotype 2 was packaged in 293T cells using the pHelper and pRC2-mi342 plasmids (Takara cat# 6652). .. A 10-cm plate of 293T cells was transfected with 5 μg pHelper, 5 μg pRC-mi342, and 5 μg LSL-3xHA KRAS targeting vector plus 45 μL PolyJet transfection reagent (SignaGen cat# SL100688).



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